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A comparative study of coastal and clinical isolates of Pseudomonas aeruginosa BJM
Nair,Anusree V.; Joseph,Neetha; Krishna,Kiran; Sneha,K. G.; Tom,Neenu; Jangid,Kamlesh; Nair,Shanta.
Pseudomonas aeruginosa is a ubiquitous Gram-negative bacterium having a versatile metabolic potential and great ecological and clinical significance. The geographical distribution of P. aeruginosahas revealed the existence of an unbiased genetic arrangement in terrestrial isolates. In contrast, there are very few reports about P. aeruginosa strains from marine environments. The present work was aimed at studying the distribution of P. aeruginosa in coastal waters along the Indian Peninsula and understanding the environmental influence on genotypic, metabolic and phenotypic characteristics by comparing marine and clinical isolates. Of the 785 marine isolates obtained on selective media, only 32 (~4.1%) were identified as P. aeruginosa, based on their fatty...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Biogeography; FAME profiling; Arbitrarily primed PCR; Environmental distribution.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300725
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Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India BJM
Parvathi,Ammini; Krishna,Kiran; Jose,Jiya; Joseph,Neetha; Nair,Santha.
Bacillus species constitute a diverse group of bacteria widely distributed in soil and the aquatic environment. In this study, Bacillus strains isolated from the coastal environment of Cochin, India were identified by detailed conventional biochemical methods, fatty acid methyl ester (FAME) analysis and partial 16S rDNA sequencing. Analysis of the data revealed that Bacillus pumilus was the most predominant species in the region under study followed by B. cereus and B. sphaericus. The B. pumilus isolates were further characterized by arbitrarily primed PCR (AP-PCR), antibiotic sensitivity profiling and PCR screening for known toxin genes associated with Bacillus spp. All B. pumilus isolates were biochemically identical, exhibited high protease and lipase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus pumilus; FAME; 16S rDNA; CesB; AP-PCR.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200012
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